FIG. 2.

Kinetics of initiation of the antiviral activities of five different HIV-1 PIs. Transfected HeLa cells (A) or infected PBMCs (B) were treated with 5 μM concentrations of each of the indicated PIs. Before treatment, all cells were trypsinized to remove unaffected, infectious virions. The infectivity of the newly assembled virions, produced in the presence of a PI, was determined by single-cycle tritration on P4 cells at different time intervals and is expressed as a percentage of the result for an untreated control. The results are means from three independent experiments with HeLa cells and two independent experiments with PBMCs.