Table 2.
LOD and LOQ of the KoP1 event-specific ddPCR assay.
| Template Amounts (HGE/Per Reaction) |
Accept Droplets | Tested HGE Copies | SD | RSD | Bias | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Rep 1 | Rep 2 | Rep 3 | Rep 1 | Rep 2 | Rep 3 | Mean | |||||
| 4500 | 13,025 | 12,904 | 12,867 | 3940 | 4260 | 4300 | 4166.67 | 197.32 | 4.74% | −7% | |
| 900 | 15,641 | 14,028 | 14,350 | 956 | 842 | 816 | 871.33 | 74.47 | 8.55% | −3% | |
| 180 | 12,585 | 13,493 | 12,323 | 186 | 174 | 188 | 182.67 | 7.57 | 4.15% | 1% | |
| 36 | 13,313 | 13,008 | 15,371 | 40 | 40 | 46 | 42.00 | 3.46 | 8.25% | 17% | |
| 7.2 | 14,878 | 14,432 | 13,456 | 6.6 | 6.2 | 8.4 | 7.07 | 1.17 | 16.58% | −2% | |
| 1.44 | 13,718 | 13,506 | 13,241 | 3.2 | 3.4 | 3.6 | 3.40 | 0.20 | 5.88% | 136% | |
| 0.29 | / | / | / | / | / | / | / | / | / | / | / |
“/” means no positive results and not detectable.