Table 3.
Dynamic range and repeatability evaluation of the event-specific ddPCR assay.
| Template Amounts (HGE/per Reaction) |
Tested HGE Copies | SD | RSD | Bias | |||
|---|---|---|---|---|---|---|---|
| Rep1 | Rep2 | Rep3 | Average | ||||
| 16,000 | 16,640 | 16,220 | 15,480 | 16,113.33 | 587.31 | 3.64 | 0.71 |
| 1600 | 1480 | 1580 | 1640 | 1566.67 | 80.83 | 5.16 | −2.08 |
| 160 | 138 | 144 | 154 | 145.33 | 8.08 | 5.56 | −9.17 |
| 50 | 62 | 58 | 50 | 56.67 | 6.11 | 10.78 | 13.33 |
| 25 | 22 | 22 | 19 | 21.00 | 1.73 | 8.25 | −16.00 |
| 10 | 14 | 12 | 9 | 11.67 | 2.52 | 21.57 | 16.67 |
| 7.2 | 6.9 | 8.2 | 7.8 | 7.63 | 0.67 | 8.72 | 6.02 |
| 5 | 2.3 | 8.6 | 3.1 | 4.67 | 3.43 | 73.50 | −6.67 |
| 1 | / | / | / | / | / | / | / |
“/” means no positive results and no detectable.