Table 4.
Quantification of the GM KoP1 contents of four simulated blood DNA samples with ddPCR assays.
| Sample (GM Content, %) |
Assay | Accept Droplets | Tested HGE Copies | SD | RSD | GM Content | Bias | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Rep 1 | Rep 2 | Rep 3 | Rep 1 | Rep 2 | Rep 3 | Average | ||||||
| B1 (1.7%) | KoP1 | 14,980 | 14,900 | 12,480 | 138 | 138 | 132 | 136.00 | 3.46 | 2.55% | 1.71% | 0.59% |
| PRLR | 10,643 | 10,877 | 11,500 | 7620 | 8100 | 8180 | 7966.67 | 302.88 | 3.80% | |||
| B2 (0.85%) | KoP1 | 16,542 | 15,859 | 12,803 | 62 | 65 | 74 | 67.00 | 6.24 | 9.32% | 0.86% | 1.18% |
| PRLR | 11,854 | 10,364 | 11,925 | 7520 | 7500 | 8280 | 7766.67 | 444.67 | 5.73% | |||
| B3 (0.5%) | KoP1 | 11,069 | 16,058 | 11,970 | 46 | 44 | 40 | 43.33 | 3.06 | 7.05% | 0.55% | 10.00% |
| PRLR | 11,854 | 11,925 | 11,500 | 7690 | 7780 | 7960 | 7810.00 | 137.48 | 1.76% | |||
| B4 (0.25%) | KoP1 | 11,441 | 13,213 | 13,213 | 20 | 24 | 22 | 22.00 | 2.00 | 9.09% | 0.28% | 12.00% |
| PRLR | 12,764 | 11,642 | 12,850 | 7910 | 7790 | 8040 | 7913.33 | 125.03 | 1.58% | |||