Table 5.
Detection of practical blood, fecal, and soil samples using the developed event-specific ddPCR assay.
| Sample Type | Sample Name | Animal Type | KoP1 Event-Specific Assay | Goat Species Assay | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Tested Copies Per Reaction | SD | RSD | Tested Copies Per Reaction | SD | RSD | |||||||||
| Rep 1 | Rep 2 | Rep 3 | Mean | Rep 1 | Rep 2 | Rep 3 | Mean | |||||||
| Milk | 46 | GM | 1340 | 1340 | 1360 | 1346.7 | 11.55 | 0.86% | 1568 | 1610 | 1520 | 1566.0 | 45.03 | 2.88 |
| 323 | GM | 984 | 1056 | 1020 | 1020.0 | 36.00 | 3.53% | 1010 | 1050 | 1028 | 1029.3 | 20.03 | 1.95 | |
| 1606 | GM | 910 | 958 | 890 | 919.3 | 34.95 | 3.80% | 906 | 942 | 930 | 926.0 | 18.33 | 1.98 | |
| 1608 | GM | 820 | 830 | 848 | 832.7 | 14.19 | 1.70% | 804 | 796 | 782 | 794.0 | 11.14 | 1.40 | |
| Fresh Feces | F1 | GM | N | N | N | / | / | / | N | N | N | / | / | / |
| F2 | GM | N | N | N | / | / | / | N | N | N | / | / | / | |
| F3 | GM | N | N | N | / | / | / | N | N | N | / | / | / | |
| F4 | GM | N | N | N | / | / | / | N | N | N | / | / | / | |
| F5 | GM | 12 | 8 | N | 10 | 2.83 | 28.30% | 14 | 12 | 9 | 11.7 | 2.52 | 21.57 | |
| F6 | GM | N | N | N | / | / | / | N | N | N | / | / | / | |
| F7 | GM | N | N | N | / | / | / | N | N | N | / | / | / | |
| F8 | GM | N | N | N | / | / | / | N | N | N | / | / | / | |
| F9 | GM | 11 | 24 | 8 | 14.3 | 8.5 | 59.34% | 8 | 6.4 | 4.6 | 6.3 | 1.70 | 26.86 | |
| F10 | GM | N | N | N | / | / | / | N | N | N | / | / | / | |
| F11 | GM | N | N | N | / | / | / | N | N | N | / | / | / | |
| F12 | GM | N | N | N | / | / | / | N | N | N | / | / | / | |
| F13 | GM | N | N | N | / | / | / | N | N | N | / | / | / | |
| F14 | GM | N | N | N | / | / | / | N | N | N | / | / | / | |
| F15 | GM | N | N | N | / | / | / | N | N | N | / | / | / | |
| F16 | GM | N | N | N | / | / | / | N | N | N | / | / | / | |
| F17 | Non-GM | N | N | N | / | / | / | 20.8 | 24.6 | 16 | 20.5 | 4.31 | 21.06 | |
| F18 | Non-GM | N | N | N | / | / | / | N | N | N | / | / | / | |
| Compost Soil | P1 | GM | N | N | N | / | / | / | N | N | N | / | / | / |
| P2 | GM | N | N | N | / | / | / | N | N | N | / | / | / | |
| P3 | GM | N | N | N | / | / | / | N | N | N | / | / | / | |
| P4 | GM | N | N | N | / | / | / | N | N | N | / | / | / | |
| P5 | GM | N | N | N | / | / | / | N | N | N | / | / | / | |
“N” means negative result; “/” means no data.