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. 2022 Mar 15;23(6):3152. doi: 10.3390/ijms23063152

Figure 2.

Figure 2

Regulation of SREBP-1 and PPAR-γ in C. acnes-induced acne model by melittin. The representative IHC analysis images showed that the expression of lipogenesis markers SREBP-1 and PPAR-γ was inhibited by the administration of melittin in a C. acnes-induced acne model. (A) The graphs indicate the relative percentage of the expression of SREBP-1 and PPAR-γ in the ear skin sections. The integrated optical densities were measured from at least five random fields per section. (B,C) The integrated optical densities were measured from at least five random fields per section. (D) The Western blot analysis shows the protein expressions of SREBP-1, PPAR-γ, and GAPDH in the ear skin of mice in each group. GAPDH was used to confirm equal sample loading. The bar graph shows the quantitative signal intensity of (E) SREBP-1 and (F) PPAR-γ after normalization with GAPDH. Histological examinations were performed at 400× magnification under light microscopy. The results are expressed as means ± SEM. * p < 0.05 compared with the NC group. † p < 0.05 compared with the CA group. NC: normal control; CA: C. acnes; M1, M10, and M100: 1, 10, and 100 μg of melittin mixed with Vaseline.