Figure 4.

Melittin inhibits IGF-1-induced lipogenic factors in SZ95 cells. (A) Cytotoxic effects of melittin on SZ95 cells. Cell viability was determined by CCK-8 assay (n = 5). (B) The effects of melittin on the expressions of lipogenic factors, such as ACC, FAS, SCD-1, and p-p70s6k in SZ95 cells that were stimulated by IGF-1. (C–F) We quantified the protein levels next. β-actin was presented as loading control. Intracellular lipids were detected by Nile red staining. (G) Representative Nile Red stain images were detected in intracellular lipids at 400× magnification. Scale bar = 50 μm. The results are expressed as means ± SEM of three independent determinations. * p < 0.05 compared with the NC group. NC group. † p < 0.05 compared with the IGF-1 group. NC: normal control; IGF-1: insulin-like growth factor-1; M0.1, M0.5, and M1: 0.1, 0.5, and 1 μg·mL⁻¹.