Figure 6.

Melittin effectively inhibits the pro-inflammatory cytokine in IGF-1-treated SZ95 cells. (A) Representative cropped Western blot analysis images show that melittin inhibited the IGF-1-induced protein expressions of p38 MAPK and pro-inflammatory cytokines, such as TNF-α, IL-1β, IL-8, and IFN-γ. β-actin was presented as a loading control. (B–F) We quantified the protein levels next. The results are expressed as means ± SEM. * p < 0.05 compared with the NC group. † p < 0.05 compared with the IGF-1 group. NC: normal control; IGF-1: insulin-like growth factor-1; M0.1, M0.5, and M1: 0.1, 0.5, and 1 μg·mL⁻¹.