Table 1.
Basic properties of Col1a2GFP clones selected for this study
| Clone | Col1a2 genotype | Homotrimer secretion | Mineralization | Hspa5/Cryab/Ddit3 upregulationa | SP7/Bglap expression |
|---|---|---|---|---|---|
| MC3T3 | + / + | − | + | − | + |
| Col1a2GFP-A | GFP/ + | + | + | ± | + |
| Col1a2GFP-B | GFP/ + | + | − | ± | + |
| Col1a2GFP-C | GFP/ + | − | − | ± | + |
| Col1a2GFP-D | GFP/ + | − | − | NDb | NDb |
| Col1a2GFP-E | GFP/- | + | + | ± | + |
| Col1a2GFP-F | GFP/ + | − | + | − | + |
aThe ± sign indicates a statistically significant yet relatively small (< two-fold) increase in just one of these three markers of cell stress in osteoblasts (Hspa5 in clone E, Cryab in clones B,C, and Ddit3 in clone A)
bND not determined. The cells had significantly altered morphology and were discarded after the mineralization experiment