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. 2022 Mar 20;14(6):1308. doi: 10.3390/nu14061308

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of EF-2001 on immunoblot for the protein level of C/EBP-α and PPAR-γ in HFD-induced adipose tissues. Male rats were divided into SD or HFD, and rats were orally administered with refined water, 3 mg/kg, or 30 mg/kg EF-2001 for 6 weeks (n = 6 rats/group). White adipose tissues containing epididymal fat were collected and separated into lipid and protein. C/EBP-α and PPAR-γ were detected by immunoblotting. (A) The protein levels of C/EBP-α and PPAR-γ were standardized depending on the amount of β-actin. The relative protein expression levels of C/EBP-α (B) and PPAR-γ (C) are shown in the graphs. Data are represented as the mean ± SEM. (n = 6); * p < 0.05 vs. control (without EF-2001 in HFD-induced obese rats).