Fig. 3.

Loss of neutral ceramidase (nCDase) decreases inflammatory cytokine and chemokine production in the kidney following cisplatin treatment. Mice were euthanized 72 h following 20 mg/kg intraperoneal cisplatin or vehicle treatment. mRNA expression relative to control gene beta-2-microglobulin of (A) tumor necrosis factor-α (Tnfα), (B) interleukin-6 (Il-6), (C) monocyte chemotactic protein-1 (Mcp-1), and (D) chemokine (C-X-C) ligand-1 (Cxcl1) were measured via real-time qRT-PCR. WT indicates wild-type mice and KO indicates nCDase^−/− mice. Statistical differences were determined by two-way ANOVA followed by Tukey post-test. ∗Statistically different than vehicle-treated WT mice. #Statistically different than cisplatin-treated WT mice. ˆStatistically different than vehicle-treated KO mice. Data expressed as mean ± SEM, n = 5–10.