Fig. 6.

Chloroquine inhibits autophagy induction in cisplatin-treated neutral ceramidase knockout (nCDase^−/−) mice. A: Western blot analysis was performed to assess relative basal protein levels of the indicated proteins in the renal cortex of nCDase^−/− and wild-type mice. n = 2. The same protein lysates and loading control gene were used for Figures 4A and 5. B: Mice were euthanized 72 h following treatment. Western blot analysis was performed to assess relative protein levels of the indicated proteins in the renal cortex of nCDase^−/− mice. n = 3–4. VEH indicates vehicle-treated nCDase^−/− mice, CQ indicates chloroquine-treated nCDase^−/− mice, CIS indicates cisplatin-treated nCDase^−/− mice, and CIS+CQ indicates cisplatin and chloroquine–treated nCDase^−/− mice. The same protein lysates and loading control gene were used for Figure 9A. C: Quantification of p62 expression relative to tubulin. D: Quantification of LC3II expression relative to tubulin.