Fig. 7.

Chloroquine exacerbates cisplatin-induced AKI in neutral ceramidase knockout (nCDase^−/−) mice. Mice were sacrificed 72 h following treatment. A: Blood urea nitrogen (BUN) was measured from plasma. B: Kidney injury molecule 1 (Kim-1) mRNA was measured by qRT-PCR from the renal cortex. C: Urinary NGAL was assessed. D: Percent body weight (BW) change from baseline was monitored each day after injection. VEH indicates vehicle-treated nCDase^−/− mice, CQ indicates chloroquine-treated nCDase^−/− mice, CIS indicates cisplatin-treated nCDase^−/− mice, and CIS+CQ indicates cisplatin and chloroquine–treated nCDase^−/− mice. Statistical differences were determined by a two-way ANOVA followed by a Tukey post-test. ∗Statistically different than vehicle-treated mice. #Statistically different than cisplatin-treated mice. ˆStatistically different than chloroquine-treated mice. Data expressed as mean ± SEM, n = 5–10.