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. 2022 Jan 21;63(3):100172. doi: 10.1016/j.jlr.2022.100172

Fig. 7.

Fig. 7

PLIN5 interacts with mitochondrial protein complexes involved in oxidative phosphorylation and mitochondrial dynamics. A: Scatterplot showing the relative intensity difference of proteins copurified with FLAG-PLIN5 and FLAG-β-Gal against the input intensities determined from the cell lysates. Every data point represents the average of two or three label-free quantifications from three biological replica experiments. Blue open circles: proteins. Green closed circles: GO annotated mitochondrial proteins. PLIN5, VDAC2, OPA1, ATGL, and SNAP23 are indicated as red closed circles. B: As in (A), label-free quantitative comparison of FLAG-PLIN5 versus FLAG-PLIN5(Δ461–463) coimmunoprecipitation experiments. About 289 proteins that copurify in two or more biological replica experiments with FLAG-PLIN5 but are not found with FLAG-PLIN5(Δ461–463) lacking the C-terminal Leu-Asp-Phe (LDF) sequence are boxed. C: GO term enrichment of proteins ranked by FLAG-PLIN5—FLAG-PLIN5(Δ461–463) intensity difference. Fold enrichment is displayed for top significant component categories. False discovery rate (FDR) is color coded. Cp = complex. GO: 0031301, integral component of organelle membrane; GO: 0098573, intrinsic component of mitochondrial membrane; CL: 22328, GO: 0070469, respirasome; GO: 0031227, intrinsic component of endoplasmic reticulum membrane; CL: 22327, GO: 0006119, oxidative phosphorylation; CL: 22331, GO: 0032981, complex I biogenesis; GO: 0005746, mitochondrial respirasome; GO: 0005770, late endosome; GO: 0031968, organelle outer membrane; GO: 0005747, mitochondrial respiratory chain complex I; GO: 0005741, mitochondrial outer membrane; CL: 22642, GO: 006617, mitochondrial protein import, and MICOS complex; CL: 22641, GO: 0008535, mitochondrial protein import, and respiratory chain complex IV assembly. D: Selected PLIN5 LDF-dependent interaction partners: Protein subunits of enriched complexes detected in FLAG-PLIN5(wt) but not FLAG-PLIN5(Δ461–463) co-IP experiments. Border colors: red, found in three, orange found in two, and yellow found in one of three replica experiments. MICOS, mitochondrial contact site and cristae organizing system; SNAP23, synaptosomal-associated protein 23; VDAC2, voltage-dependent anion-selective channel protein 2.