Fig. 2.

Comparison of rapid microplate assay to size-exclusion chromatography assay of LPL activity. Human VLDL isolated by ultracentrifugation from a single donor at 20 mg/dl (protein) in LPDP was incubated with increasing amounts of human LPL for 30 min at 37°C. The samples were then analyzed by size-exclusion chromatography on a Superose 6 column (as for Fig. 1) or put through the 96-well desalting plate protocol described in the Materials and methods section. A: Percent of TG hydrolysis by LPL as analyzed by both methods. B: Correlation plot of TG hydrolysis as measured by the microplate and size-exclusion methods. The line shows a simple linear regression (r² = 0.9708). All experiments were performed in triplicate, and error bars indicate one sample standard deviation.