Fig. 4.

SDS gel electrophoresis of VLDL particles isolated from cohort 1. Ultracentrifugally isolated VLDL obtained from each donor in Fig. 3 was loaded by equal protein on a 4–15% SDS gradient gel and run at 200 V for 30 min under reducing conditions followed by Coomassie staining. The lane numbers correspond to the donor who provided the particles. Expected protein molecular weights for common VLDL: apoB100 (MW, 480 kDa), APOE (MW, 34 kDa), and the apoCs (MW, between 6.6 and 8.8).