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MS-based proteomic analysis of ultracentrifugally isolated VLDL from each donor in cohort 1. About 50 μg of total VLDL protein from each donor was analyzed by MS after delipidation, reduction, alkylation, and digestion with trypsin as described in the Materials and methods section. Data are expressed as the label-free quantitation intensity as calculated by MaxQuant. The plotted data are an average of three separate runs and normalized to apoB concentration.
