Fig. 9.

Effect of APOE isoforms on VLDL TG hydrolysis by LPL. The experiment was performed as in Fig. 8 except that 25 μg/ml of either human plasma APOE or recombinant APOE2, APOE3, or APOE4 were incubated with the VLDL particles (total protein of 20 mg/dl) for 1 h at 37°C prior to performing an LPL TG hydrolysis assay. The same source of VLDL was used for each experiment, which were performed in triplicate with error bars representing one sample standard deviation. P < 0.001 by one-way ANOVA. ∗ indicates difference from VLDL alone, P < 0.05 by Tukey test.