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Outer membrane protein preparations. Approximately 20 μg of protein was solubilized in sample buffer with β-mercaptoethanol and boiled for 5 min. Lane A, 1026b; lane B, PMB-4; lane C, PMB-14; lane D, PMB-17; lane E, PMB-7; lane F, PMB-20; lane MW, prestained protein molecular mass standards (Gibco BRL). Arrows indicate the positions of apparent missing protein bands.
