TABLE 1.
Strains and plasmids
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| SM10 | Mobilizing strain, transfer genes of RP4 integrated in chromosome; Kanr Strs | 36 |
| SM10 λ pir | SM10 with a λ prophage carrying the pir gene | 25 |
| SURE | e14− (mcrA) Δ(mcrCB-hsdSMR-mrr)171 endA1 supE44 thi-1 gyrA96 relA1 lac recB recJ sbcC umuC::Tn5 uvrC [F′ proAB lacIqZΔM15 Tn10]; Kanr Tetr | Stratagene |
| DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17 deoR thi-1 supE44 gyrA96 relA1 | Bethesda Research Laboratories |
| B. thailandensis E264 | Environmental isolate; Kanr Genr Pmbr Strr Tetr | 5 |
| B. pseudomallei | ||
| 1026b | Clinical isolate; Kanr Genr Pmbr Strr Tets Tmps Zeos | 12 |
| DD503 | 1026b derivative; allelic exchange strain; Δ(amrR-oprA) (Kans Gens Strs); rpsL (Strr) | 28 |
| SRM 117 | 1026b derivative; wbiI::Tn5-OT182; Tetr | 13 |
| PMB-7 | 1026b derivative; waaF::Tn5-OT182; Tetr | This study |
| PMB-20 | 1026b derivative; udg::Tn5-OT182; Tetr | This study |
| PMB-4 | 1026b derivative; lytB::Tn5-OT182; Tetr | This study |
| MB100 | DD503 derivative; waaF::dhfrIIb-p15AoriV; Zeor | This study |
| MB203 | DD503 derivative; lytB::dhfrIIb-p15AoriV; Tmpr | This study |
| MB300 | DD503 derivative; udg::dhfrIIb-p15AoriV; Tmpr | This study |
| MB301 | DD503 derivative; waaE::dhfrIIb-p15AoriV; Zeor | This study |
| MB100C | MB100(pUCP28T::waaF); Strs Zeor Tmpr | This study |
| MB203C | MB203(pRK415::lytB); Strs Tmpr Tetr | This study |
| MB300C | MB300(pRK415::udg); Strs Tmpr Tetr | This study |
| Plasmids | ||
| pOT182 | pSUP102(Gm)::Tn5-OT182; Chlr Genr Ampr Tetr | 24 |
| p34E-oriTP | Vector containing self-cloning Tmpr cassette; dhfrIIb-p15AoriV | 6 |
| p34E-oriZeo | Vector containing self-cloning Zeor cassette; dhfrIIb-p15oriV | 6 |
| pPMB-7B | 4.0-kb BamHI fragment from PMB-7 obtained by self-cloning; contains partial waaF gene; Ampr Tetr | This study |
| pPMB-4Ss | 4.5-kb SstI fragment from PMB-4 obtained by self-cloning; contains partial lytB gene; Ampr Tetr | This study |
| pPMB-20H | 8.0-kb HindIII fragment from PMB-20 obtained by self-cloning; contains partial udg gene; Ampr Tetr | This study |
| pPMB-20Ss | 5.2-kb SstI fragment from PMB-20 obtained by self-cloning; contains waaE gene; Ampr Tetr | This study |
| pUCP28T | Broad-host-range vector; IncP OriT; pRO1600ori; Tmpr | 34 |
| pRK415 | Broad-host-range vector; Tetr | 22 |