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. 2022 Feb 23;20(3):159. doi: 10.3390/md20030159

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Degradation products analysis of the alginate lyases of strain M9. (A), SDS-PAGE analysis of the purified alginate lyases AlyM1, AlyM2, AlyM3, and AlyM5. The recombinant protein bands of the alginate lyases are indicated by arrows. (B), gel filtration chromatography analysis of the degradation products of the alginate lyases. Gel filtration chromatography analysis was performed using a Superdex peptide 10/300 GL column monitored at a wavelength of 210 nm. The control was treated with pre-heated inactivated lyases. Saturated mannuronate oligosaccharides from DP1 to DP6 were taken as the standards. DP, degree of polymerization.