Structure comparison of AlyM2 with other PL6 alginate lyases. (A), comparison of the overall structure of AlyM2 with other PL6 structures. AlyM2 structure was predicted by Alphafold2. Structures of AlyF (Genbank accession number: WP_116871951.1; PDB code: 6ITG) and AlyGC (Genbank accession number: WP_007984897.1; PDB code: 5GKQ) are representative PL6 structures. (B), the arrangement of the key residues in the active centers of the PL6 alginate lyases. The amino acid residues are shown in sticks. AlyM2 is in orange, Patl_3640 (PDB code: 7O77) in silver, Pedsa_3628 (PDB code: 7O79) in blue, Pedsa_0632 (PDB code: 7O7A) in salmon, AlyGC (PDB code: 5GKD) in green, AlyF (PDB code: 5Z9T) in cyan, BcelPL6 (PDB code: 6QPS) in magenta, and BcAlyPL6 (PDB code: 7DML) in yellow.