TABLE 4.
Yield of cells and ergosterol content of a 100-ml culture under stationary growth conditions
| Method (units of measurement) | Avg yield ± SDa (itraconazole IC50 [μM])
|
||
|---|---|---|---|
| C43 | C48 | C56 | |
| Coulter counter (108 cells) | 201 ± 24 (0.03) | 127 ± 10b (0.07) | 111 ± 6b (0.1) |
| UV analysis (μg of Δ5,7-diene sterol) | 1,068 ± 125 (0.02) | 1,049 ± 169 (0.1) | 921 ± 81 (0.12) |
| Sterol/cell ratio (fg/cell) | 53 ± 8 | 82 ± 9 | 83 ± 9 |
a
Based on at least six measurements.
b
P < 0.05 as determined by Wilcoxon statistical analysis.