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Kinetic analysis of recombinant β-D-xylosidases from L. reuteri and B. producta strains using pNP-Xyl as substrate, determined in 50 mM sodium phosphate buffer (pH 5.7) at 45 °C.
| Enzyme | Km (mM) | kcat (s−1) | kcat/Km (s−1mM−1) |
|---|---|---|---|
| Bp- XylX-1 | 0.19 ± 0.02 | 11.9 ± 0.23 | 63 |
| Bp-XylX-2 | 0.19 ± 0.02 | 10.7 ± 0.2 | 56 |
| Lr-XylC | 0.23 ± 0.02 | 11.4 ± 0.2 | 49 |
| Bp-XylX-1-D371 | 0 | 0 | 0 |
| Bp-XylX-1-E408 | 0 | 0 | 0 |
| Bp-XylX-2-D386 | 0 | 0 | 0 |
| Bp-XylX-2-E409 | 0 | 0 | 0 |
| LR-XylC -D382 | 0 | 0 | 0 |
| LR-XylC -E415 | 0 | 0 | 0 |
