Figure 5.

Doxorubicin-loaded microscale hydrogels reduce glioma cell viability, whilst unloaded hydrogels are not cytotoxic. PrestoBlue^® analysis of C6 glioma cell viability for 50 µm-width hydrogels (a) and 100 µm-width hydrogels (c) showed that unloaded hydrogels were not toxic at any concentration analyzed, yet, reduced cell viability in a dose-dependent manner when loaded with doxorubicin (n = 4, error bars represent ± standard deviation). Light microscope images of 50 µm-width hydrogels (b) and 100 µm-width hydrogels (d) loaded with doxorubicin, immediately after addition of the hydrogels at a concentration of 480 µg/mL and then again after 48 h. Scale bars represent 200 µm.