FIG. 2.

Inhibitory effects of EM and FK506 on transcriptional activation of cis-acting enhancer elements. Transgenic Jurkat T cells that stably express the firefly luciferase gene driven by IL-8 NF-κB (positions −84 to +44) (a) or three tandem copies of IL-2 NFAT (b) were established (see Materials and Methods). These T-cell lines were pretreated with either EM or FK506 at a dose range of 10⁻⁹ to 10⁻⁴ M for 1 h and were then stimulated with PMA (20 ng/ml) plus ionomycin (2 μM) for 4 h in the presence of the drugs. The cells were then subjected to the luciferase assay as described in the legend to Fig. 1.