Table 2.

List of the primers used for Snapback assay and amplification and sequencing of the targeted genes. The snapback tail of 16 bases is shown as underlined.

Target Gene	Primer	Amplicon Size
RPO30 Snapback	Cp-HRM-SBF-5′-ggTGTAGTACGTATAAGATTATCGTATAGAAACAAGCCTTTA-3′	SNAP BACK assay
	Cp-HRM1R-5′-AATTTCTTTCTCTGTTCCATTTG-3′
GPCR_ FRET	CpRt Forward- 5′-GATAGTATCGCTAAACAATGG-3′	200 bp
	CpRt Reverse- 5′-ATCCAAACCACCATACTAAG-3′
RPO30	Cp-OL1F 5′-CAGCTGTTTGTTTACATTTGATTTTT-3′	554 bp
	Cp-OL1R 5′-TCGTATAGAAACAAGCCTTTAATAGA-3′
	Cp-OL2F 5′-TTTGAACACATTTTATTCCAAAAAG-3′	520 bp
	Cp-OL2R 5′-AACCTACATGCATAAACAGAAGC-3′
GPCR	CpGPCR-OL1F-5′-TGAAAAATTAATCCATTCTTCTAAACA-3′	684 bp
	CpGPCR-OL1R-5′-TCATGTATTTTATAACGATAATGCAAA-3′
	CpGPCR-OL2F-5′-TTAGCGGTATAATCATTCCAAATA -3′	603 bp
	CpGPCR-OL2R-5′-GCGATGATTATGATGATTATGAAGTG-3‘
	CpGPCR-OL3F-5′-CACAATTATATTTCCAAATAATCCAA -3′	617 bp
	CpGPCR-OL3R-5′-TGTACATGTGTAATTTTAATGTTCGTA-3′
EEV glycoprotein gene (ORF LSDV126)	EEVGly F- 5′-ATGGGAATAGTATCTGTTGTATACG-3′	250 bp
	EEVGly R-5′-CGAACCCCTATTTACTTGAGAA-3′
B22R	B22R F- 5′-TCATTTTCTTCTAGTTCCGACGA-3′	863 bp
	B22R R- 5′-TTCGTTGATGATAAATAACTGGAAA-3′