Table 3.
PCR-based snap back assay results for detection and genotyping of LSDV.
| S. No. | Sample ID | Host | SNAP BACK Assay Analysis | Remarks | ||
|---|---|---|---|---|---|---|
| Cq | Tm1 (°C) | Tm2 (°C) | ||||
| 1 | K/B/Sb/1/2020 | Buffalo | N/A | N/A | N/A | Negative |
| 2 | K/B/Sb/2/2020 | Buffalo | N/A | N/A | N/A | Negative |
| 3 | T/C/Sb/3/2020 | Cattle | 20.18 | 51.00 | 74.00 | LSDV |
| 4 | Kt/C/NS/4/2020 | Cattle | 28.73 | 50.50 | 74.00 | LSDV |
| 5 | Kt/C/NS/5/2020 | Cattle | N/A | N/A | N/A | Negative |
| 6 | Bkt/C/Sb/6/2020 | Cattle | 24.25 | 51.00 | 74.00 | LSDV |
| 7 | P/C/S/7/2020 | Cattle | 37.38 | 50.50 | 73.50 | LSDV |
| 8 | Kr/B/S/8/2020 | Buffalo | 24.86 | 50.00 | 73.50 | LSDV |
| 9 | S/C/Sb/9/2020 | Cattle | 22.48 | 51.00 | 74.00 | LSDV |
| 10 | Sn/C/Sb/10/2020 | Cattle | 19.83 | 51.00 | 74.00 | LSDV |
| 11 | S/C/NS/11/2020 | Cattle | 24.83 | 51.00 | 74.00 | LSDV |
| 12 | L/C/T/12/2020 | Cattle | 25.14 | 51.00 | 74.00 | LSDV |
| 13 | R/C/OS/13/2020 | Cattle | N/A | N/A | N/A | Negative |
| 14 | N/C/S/14/2020 | Cattle | N/A | N/A | N/A | Negative |
| 15 | Sn/B/S/15/2020 | Buffalo | 36.34 | 51.00 | 73.50 | LSDV |
| 16 | K/C/S/16/2020 | Cattle | N/A | N/A | N/A | Negative |
| 17 | B/C/Sb/17/2020 | Cattle | 24.97 | 51.00 | 74.00 | LSDV |
| 18 | B/C/Sb/18/2020 | Cattle | 29.28 | 50.50 | 74.00 | LSDV |
| 19 | D/C/T/19/2020 | Cattle | 31.02 | 50.00 | 73.50 | LSDV |
| 20 | D/C/T/20/2020 | Cattle | 22.87 | 50.50 | 74.00 | LSDV |
| GTPV | GTPV positive control | N/A | 27.82 | 56.00 | 72.50 | GTPV |
| SPPV | SPPV positive control | N/A | 27.88 | 52.00 | 72.50 | SPPV |
| LSDV | LSDV positive control | N/A | 24.90 | 50.50 | 73.50 | LSDV |
| NTC | RNAse free water | N/A | N/A | N/A | N/A | Negative |