FIG. 4.
Dose dependence of ANS-G on inhibition of VSV replication. Cells (0.125 × 106 cells/well) were treated with various concentrations of ANS-G in 250 μl of serum-free RPMI 1640 for 4 h at 37°C. The cells were then infected with VSV at a MOI of 0.5 for 30 min after which they were given three rapid washes. The cells were incubated at 37°C for 10 h postinfection in medium containing the oligonucleotides with 2% FBS. Culture supernatants were collected, and viral titers were estimated by plaque assays of CHO cell monolayers. Results shown are means ± standard errors of three independent determinations.