TABLE 1.
Oligonucleotide sequencesa
| Oligomer | Sequence |
|---|---|
| ANS | CAT TTT GAT TAC TGT |
| sANS | CAT TTT GAT TAC TGT |
| cANS | CAT TTT GAT TAC TGT |
| Sense | ACA GTA ATC AAA ATG |
| Scram | ATC GTA TTA CTT GTT |
| ANS-G | CAT TTT GAT TAC TGT GGG GGG GGG G |
| sANS-G | CAT TTT GAT TAC TGT GGG GGG GGG G |
| cANS-G | CAT TTT GAT TAC TGT GGG GGG GGG G |
| Sense-G | ACA GTA ATC AAA ATG GGG GGG GGG G |
| Scram-G | ATC GTA TTA CTT GTT GGG GGG GGG G |
| ANS-C | CAT TTT GAT TAC TGT CCC CCC CCC C |
a
The 15-mer antisense oligonucleotide (ANS) was directed against the translation initiation site of the VSV N protein mRNA. sANS, PS analog of ANS; cANS, chimeric antisense oligomer with the first and last internucleotide linkages modified by PS chemistry; sense, control sequence complementary to ANS; scram, scrambled control sequence with the same composition as ANS; ANS-G, sANS-G, cANS-G, sense-G, and scram-G are the respective oligonucleotides, each containing a 10-mer poly(G) tail at the 3′ end; ANS-C, control oligonucleotide sequence with ANS linked to a 10-mer poly(C) tail at the 3′ end. The internucleotide linkages for underlined sequences are linked through PS bonds.