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. 2022 Mar 11;23(6):3059. doi: 10.3390/ijms23063059

Figure 3.

Figure 3

The region with the predicted NLS provides functions in addition to the nuclear localization signal. (A) Schematic illustration of the different PAP8 recombinant genes. cTP, chloroplast transit peptide; NLS, nuclear localization signal; m5, five substitutions leading to amino acid changes of K and R in G; dhc, double hemi-complementation test. (B) PCR-based genotyping of the different lines showing in particular that dhc possess the two constructions (Nm5 and ΔcTP). (C) Dot plot of the percentage of the delayed greening phenotype obtained in the segregating offspring from the doubly heterozygous plants from dhc (light orange dots) or independent T1 for BB647 (light green dots). A ratio of 1/4 (25%) corresponds to absence of genetic interaction between the transgene and the pap8-1 allele: no complementation. (ε-test, α = 0.05; Fisher Yates) dhc #04: 44/148 (ε = 1.26); #19: 115/543 (ε = 2.179); #22: 63/234 (ε = 0.663); #33: 83/292 (ε = 1.297); #36: 44/149 (ε = 1.212); #40: 15/47 (ε = 1.017); #46: 98/373 (ε = 0.558) sum of all is 462 [Albino] over 1324 [WT] εsomme = 0.838 < 𝒰5% = 1.96. BB647 #Na: 79/279 (ε = 1.229); #Za: 91/365(ε = 0.03); #Ka: 53/235 (ε = 0.897); #Ab: 91/366 (ε = 0.06); #Ec: 73/314 (ε = 0.734); #Lb: 84/340 (ε = 0.125); #Ob: 65/279 (ε = 0.672); #Ub: 43/212 (ε = 1.708); #Vb: 55/229 (ε = 0.348); #Gc: 56/269 (ε = 1.689); #41: 65/266 (ε = 0.214); sum: 755/3154 (εsomme = 1.397 < 𝒰5% = 1.96). (D) 7-day-old (7 DO) or (E) 30-day-old (30 DO) phenotype of the given genotypes.