Figure 1.

Acute endotoxemia affects adipose tissue but does not lead to weight gain in mice under chow diet. Swiss Webster mice were submitted to intraperitoneal administration of 8 consecutive doses of 10 mg/kg LPS, every 3 days. (a) Endotoxin and (b) SAA concentration in serum. (c) Daily caloric intake. (d) Weight gain curve of control and LPS mice. Vertical dashed line in c and d indicate end of acute endotoxemia period. (e) Epididymal fat pad weight. (f) Epididymal adipocyte size. (g–k) Quantitative real-time PCR was performed to assess mRNA expression of (g) Saa1.1/2.1, (h) Saa3, (i) Tlr-2, (j) Tlr-4 and (k) Cd14 in epididymal adipose tissue. (l) Histological sections of epididymal fat pads after LPS challenges showing adipocyte morphology on hematoxylin and eosin staining and macrophage infiltration (F4/80⁺). Statistical analysis; for all variables with the same letter (a, b or c), the difference between the means is not statistically significant. Where two variables have different letters (a, b or c), they are significantly different (p < 0.05). Data are means ± SEM from 6 mice per group (* p < 0.05, ** p < 0.01, *** p < 0.001, between groups, as indicated).