Table 1.
Intra- and interday precision and accuracy of the assay.
| Nominal Concentration (ng/mL) |
Measured Concentration (ng/mL) |
Accuracy (%RE) | Precision (%CV) |
|---|---|---|---|
| Rat plasma | |||
| Intraday (n = 6) | |||
| 2 | 2.107 ± 0.265 | 5.333 | 12.60 |
| 5 | 4.900 ± 0.306 | 2.000 | 6.240 |
| 100 | 103.1 ± 9.426 | 3.083 | 9.144 |
| 2500 | 2608 ± 196.1 | 4.333 | 7.518 |
| Interday (n = 18) | |||
| 2 | 2.000 ± 0.199 | 0.139 | 9.972 |
| 5 | 5.290 ± 0.385 | 5.700 | 7.278 |
| 100 | 110.6 ± 8.540 | 10.61 | 7.721 |
| 2500 | 2649 ± 202.9 | 5.956 | 7.662 |
| Mouse plasma | |||
| Intraday (n = 6) | |||
| 2 | 2.047 ± 0.257 | 2.333 | 12.55 |
| 5 | 4.867 ± 0.370 | 2.667 | 7.603 |
| 100 | 99.20 ± 8.536 | 0.850 | 8.609 |
| 2500 | 2513 ± 249.6 | 0.533 | 9.932 |