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. 2022 Mar 8;11(6):715. doi: 10.3390/plants11060715

Figure 4.

Figure 4

The analysis of NPTII mRNA levels in Arabidopsis thaliana in response to external application of synthetic NPTII-encoding D1 and D1Me DNA oligonucleotides designed to target the 5′ end of the transgene. (a) Foliar application of unmethylated single-stranded DNA oligonucleotides (D1-s, D1-a) and siRNA-mimicking DNA duplexes (D1-s+a). (b) Foliar application of methylated single-stranded DNA oligonucleotides (D1-s, D1-a) and siRNA-mimicking DNA duplexes (D1-s+a). WC—A. thaliana treated with sterile water (100 µL per plant); The NPTII mRNAs were measured 1 day and 7 days post-treatment. The DNA oligonucleotides were diluted in nuclease-free water to 50 pmol/μL (100 μL per plant). KA0-1 and KA0-2—transgenic Arabidopsis lines bearing the NPTII transgene under the control of the doubled CaMV 35S promoter. qRT-PCR data are presented as mean ± SE (three independent experiments). Means on each figure followed by the same letter were not different using one-way analysis of variance (ANOVA), followed by the Tukey HSD multiple comparison test.