Table 2.

List of primers and thermocycling conditions.

Selection Method	Primer	Sequence	PCR Product Size	Thermocycling Conditions
K1L	TKL new	GCTACCACCGCAATAGATCCT	897 bp for wild-type MVA; 2910 bp for rMVA-K1L	Initial denaturation at 95° for 5 min; followed by 40 cycles at 95° for 30 s, 55° for 30 s, 72° for 4 min; and a final extension at 72° for 5 min.
	TKR new	CTAATATACCGTGTCGCTGTAAC
F13L	a49	CCTCAGTTTCAATATCTCCTTCCTG	2539 bp for wild-type MVA; 2322 bp for MVA ΔF13L; 3095 bp for rMVA F13Lrev	Initial denaturation at 95° for 5 min; followed by 40 cycles at 95° for 30 s, 52° for 30 s, 72° for 4 min; and a final extension at 72° for 5 min.
	a50	GTTTTTATATGTGTTTGATCTAACGAG
D4R	a58	ACCTTCCAACTGTGGATACTCTG	2430 bp for wild-type MVA; 2625 bp for MVA ΔD4R; 2960 bp for rMVA D4Rrev	Initial denaturation at 95° for 5 min; followed by 40 cycles at 95° for 30 s, 52° for 30 s, 72° for 4 min; and a final extension at 72° for 5 min.
	a59	TCGAATGAAATAAACCCTGGT