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Effects of UTP on [Ca2+]i concentrations in human differentiated white adipocytes. Human adipocytes, grown and differentiated in adipogenic medium on glass coverslips, were loaded with fura-2/AM as described in the Section 4. (A) Adipocytes suspended in Ca2+-containing medium. (B) Adipocytes suspended in Ca2+-free medium. Where indicated, UTP (100 μM) and Ca2+ (1 mM) was added. Traces are representative of the fluorescence ratio F340/F380 of a typical experiment of three.
