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. 2022 Mar 16;27(6):1913. doi: 10.3390/molecules27061913

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Stimulation with ATP inhibits the effects of UTP on [Ca²⁺]_i concentrations in human differentiated white adipocytes. (B) Stimulation with UTP does not inhibit the effects of ATP on [Ca²⁺]_i concentrations in human differentiated white adipocytes. Human adipocytes, grown and differentiated in adipogenic medium on glass coverslips, were loaded with fura-2/AM, as described in the Section 4. Where indicated, ATP (100 μM) and UTP (100 μM) were added. Traces are representative of the fluorescence ratio F340/F380 of a typical experiment from three.