Table 1.

List of primer sequences and experimental conditions in PCR.

Gene	Forward (5′-3′)	Reverse	Annealing Temperature (°C)	N. of Cycle	Expected Product (bp)	Positive Control
P2X1	TTCAGGTTTGCCAGGCACTTT	CCCCAAAGATGCCAATTCCA	59	40	160	PBMC
P2X2	GGTGTCATCGGGGTCATTAT	ACCTGAAGTTGTAGCCGTACGA	56	40	130	A-172
P2X3	CACCTATGAGACCACCAAGTCG	CGTCACGTAATCAGACACATCC	59	40	222	A-172
P2X4	TGTGATACCAGCTCAGGAGGAAAAC	GCATCATAAATGCACGACTTGAGGT	66	35	396	PBMC
P2X5	CTTCTCCAAAAGCAATGTGATGGAC	GATGAGTACCAGGTCGCAGAAGAAA	65	39	406	PBMC
P2X6	ATGGAATCCGCTTCGACATC	TCCTCCAGTAGAAATGGGCTTC	60	38	165	HEK-293
P2X7	TGATAAAAGTCTTCGGGATCCGTTT	CCTGGACAAATCTGTGAAGTCCATC	65	39	398	PBMC
P2Y1	ATGTGTGCTTTCAATGACAGGGTTT	TGTGGATGTGGGCATTTCTACTTCT	66	38	399	PBMC
P2Y2	GTGTGCATTCATGAGTGAGGAACC	ATCAGACACAGCCAGGTGGAACATA	68	40	313	PBMC
P2Y4	CCACCTGGCATTGTCAGACACC	GAGTGACCAGGCAGGCACGC	61	38	425	PBMC
P2Y6	CGCTTCCTCTTCTTGCCAACC	CCATCCTGGCGGCACAGGCGGC	62	36	365	Placenta
P2Y11	CTACAGAGCGTATAGCCTGGTGCTG	CCATGTAGAGTAGAGGGTGGACACA	70	39	365	PBMC
P2Y12	CATTCAAACCCTCCAGAATCAACAG	CGATCGATAGTTATCAGTCCCAGGAA	68	36	413	PBMC
P2Y13	AAAAACACTTTGGTGGCCGACTT	ACAGCACGATGCCCACATACAT	58	37	154	PBMC
P2Y14	CACTTCAAGACGACAAACG	GAATATCCATCCTGACACTCC	60	38	108	Placenta