TABLE 3.
MICs of quinupristin, dalfopristin, and quinupristin-dalfopristin for three recipient strains containing the ermB, satA, and vgb genes cloned in various combinations on plasmid pJIM2246
| Strain and plasmid | MIC (μg/ml) of drug(s)
|
|||
|---|---|---|---|---|
| Quinupristin
|
Dalfopristin | Quinupristin-dalfopristinb | ||
| Not induced | Induceda | |||
| E. faecium HM1070 | ||||
| pJIM2246 | 1 | 1 | 1 | 0.25 |
| pJIM2246 ΩermB | 2 | 4 | 1 | 0.25 (1) |
| pJIM2246 ΩsatA | 1 | 1 | 8 | 0.5 (2) |
| pJIM2246 Ωvgb | 8 | 8 | 1 | 1 (4) |
| pJIM2246 Ω(ermB satA) | 2 | 4 | 32 | 2 (8) |
| pJIM2246 Ω(ermB vgb) | 8 | 8 | 1 | 1 (4) |
| pJIM2246 Ω(satA vgb) | 8 | 8 | 32 | 4 (16) |
| pJIM2246 Ω(ermB satA vgb) | 16 | 16 | 32 | 4 (16) |
| E. faecium BM4107 | ||||
| pJIM2246 | 2 | 2 | >128 | 1 |
| pJIM2246 ΩermB | 4 | 32 | 128 | 1 (1) |
| pJIM2246 ΩsatA | 4 | 4 | >128 | 4 (4) |
| pJIM2246 Ωvgb | 4 | 4 | 128 | 4 (4) |
| pJIM2246 Ω(ermB satA) | 4 | 16 | >128 | 8 (8) |
| pJIM2246 Ω(ermB vgb) | 8 | 8 | 128 | 8 (8) |
| pJIM2246 Ω(satA vgb) | 8 | 8 | >128 | 16 (16) |
| pJIM2246 Ω(ermB satA vgb) | 16 | 16 | >128 | 16 (16) |
| S. aureus RN4220 | ||||
| pJIM2246 | 1 | 1 | 1 | 0.25 |
| pJIM2246 ΩermB | 2 | 16 | 1 | 0.25 (1) |
| pJIM2246 ΩsatA | 1 | 1 | 8 | 0.5 (2) |
| pJIM2246 Ωvgb | 8 | 8 | 1 | 1 (4) |
| pJIM2246 Ω(ermB satA) | 4 | 8 | 16 | 1 (4) |
| pJIM2246 Ω(ermB vgb) | 8 | 8 | 1 | 1 (4) |
| pJIM2246 Ω(satA vgb) | 8 | 8 | 16 | 4 (16) |
| pJIM2246 Ω(ermB satA vgb) | 8 | 16 | 16 | 4 (16) |
a
Induction was done by growing bacteria overnight in medium containing 1 μg of quinupristin per ml.
b
Values in parentheses are the MIC multiplication factors. The MIC multiplication factor is the ratio of the MIC for the transformant to the MIC for the recipient containing pJIM2246.