Figure 2:

HnRNP K suppressed clotrimazole-induced apoptosis of U-2OS cells. (A) Ectopic expression of hnRNP K overexpressing construct or vector only in U-2OS cells was followed by clotrimazole treatment. The expression of HA-HnRNP K constructs were assessed by Western blot analysis using anti-HA or anti-HnRNP K antibodies. (B) U-2OS cells transiently transfected with control siRNA or si hnRNP K were treated with clotrimazole. For A and B, FACS analysis using 6 biological replicates was performed to determine apoptosis level at the indicated conditions. Error bars indicate standard derivation. *P<0.01.