Figure 3.

WT and Alkbh8^Def livers have a similar transcriptional response to a single dose of APAP. mRNA-seq data for (A) Alkbh8^Def APAP vs. WT APAP was compared using an enhanced volcano plot. (B) Post-transcriptional regulation of translation by Alkbh8 and epitranscriptomic marks. Alkbh8 requires the small accessory protein Trm112 and S-adenosylmethionine (SAM) to modify the wobble position of tRNA^Sec (C) from cm⁵U to mcm⁵U, which is then further modified to the ribose-methylated derivative mcm⁵Um. (D) Stop-codon recoding utilizes mcm⁵U and mcm⁵Um in tRNA^Sec to decode UGA to produce selenoproteins. In addition to Alkbh8-catlyzed epitranscriptomic marks, elongation factors, a selenocysteine insertion sequence (SECIS), accessory proteins, and an internal UGA stop codon are used to promote the translation of selenocysteine containing proteins.