Figure 4.

Multiple RNA modifications on tRNA for selenocysteine and other tRNAs are differentially regulated in WT and Alkbh8^Def livers in response to a single dose of APAP. (A) Epitranscriptomic marks found on tRNA^Sec. (B,C) WT and Alkbh8^Def mice (N = 3) were exposed to a single dose of APAP and livers were harvested 6 h after dosing. (B) Modifications that occur throughout tRNA^Sec and other tRNAs were measured using LC-MS/MS. (C) Writer enzyme gene count changes in expression vs. p-value determined. (D) Selenoprotein levels (N = 3) were evaluated using the ProteinSimple WES system. Statistical significance was determined using an unpaired t-test with (* p < 0.05, ** p < 0.01).