Table 3.
Biological activity of blood neutrophils ex vivo. Phagocytosis mean fluorescence intensity (MIF).
| Phagocytosis (MIF) a | Netosis (RFU) b | |||||
|---|---|---|---|---|---|---|
| H | D | p-vig | H | D | p-vig | |
| Neu | 3.7 ± 0.6 | 3.7 ± 0.5 | 1397 ± 453 | 2990 ± 215 | 0.001 | |
| Neu+Sa1 | 181.7 ± 23.6 | 91.3 ± 5.1 | 0.05 | 7237 ± 577 ** | 6967 ± 511 ** | |
| Neu+LPS+Sa1 | 408.3 ± 18.6 ** | 316.7 ± 70.2 ** | 0.05 | ND | ND | |
| Neu+SLp+Sa1 | 246.3 ± 8.5 * | 219.7 ± 21.2 * | 6113 ± 944 | 5864 ± 1112 | ||
| Neu+SLpN+Sa1 | 269.3 ± 12.9 * | 218.3 ± 18.7 * | ND | ND | ||
| Neu+Lp v+Sa1 | ND | ND | 7180 ± 1182 | 6802 ± 872 | ||
| Neu+Lp c+Sa1 | ND | ND | 6203 ± 1076 | 5938 ± 1126 | ||
| Neu+Lp d+Sa1 | ND | ND | ND | ND | ||
a Results are expressed as mean ± SD. p-vig: p-value intergroup. Statistical differences between Neu+Sa1 and other assays intra-groups * (p < 0.01), ** (p < 0.001). Netosis reference fluorescent units (RFU). b Statistical differences between Neu and Neu+Sa1 ** p< 0,001. H: healthy, D: diabetic patient, ND: non-determined, Neu: blood neutrophils, v: viable L. plantarum cells, c: L. plantarum whole culture, d: death L. plantarum cells.