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. 2022 Mar 15;23(6):3178. doi: 10.3390/ijms23063178

Figure 1.

Figure 1

Immunohistochemical mapping revealed that regional distribution of dynorphinergic neurons in the brain of a Pdyn::cas9-EGFP mouse shows large similarity to the reference atlas provided by the Allen Institute. The distribution of Pdyn neurons was evaluated by plotting immunopositive cell bodies (revealed by the HRP/DAB method) through the telencephalon/diencephalon (A), the midbrain (B), and the medulla (C) of a Pdyn::cas9-EGFP mouse. On the right side of each panel, blue dots represent the location of Pdyn-immunopositive neurons, detected and reconstructed with the aid of Neurolucida (EGFP + somata). The left side of each panel shows the corresponding reference image of in situ hybridization (ISH) data from the Allen Brain Atlas (ABA) [22] displaying Pdyn mRNA expression pattern. PrMo, primary motor area; PrSs, primary somatosensory area layer 2/3 and layer 5; Dg, dentate gyrus; Thal, mediodorsal nucleus of the thalamus; Hy, dorsomedial nucleus of the hypothalamus; CP, caudoputamen; Am, central amygdalar nucleus; 3V, third ventricle; IC, inferior colliculus; PAG, periaqueductal gray; PBn, parabrachial nucleus; DR, dorsal nucleus raphe; CA, cerebral aqueduct; CER, cerebellum; nST, nucleus of the solitary tract; snTr, spinal nucleus of the trigeminal; nR, reticular nucleus. Scale bar, 500 µm. For original images, see Supplementary Figure S1.