Figure 4.

Diagnostic capacity of differentially regulated circulating miRNAs in stroke patients. The orthogonal partial-least-squares-discriminant analysis (OPLS-DA) was performed using the top differentially regulated miRNAs (n = 27) in the discovery cohort data. The classifier was trained on data from all participants in (A) discovery cohort (n = 142) and tested on the (B) validation cohort (n = 143). Scatter plots show the predictive component to discriminate stroke cases from healthy controls (green dots—x-axis) versus the orthogonal component representing a multivariate confounding effect that is independent of stroke (blue dots—y-axis). (C) ROC curve analysis generated an overall sensitivity of 0.94, specificity of 0.99, and AUC of 0.97.