Table 1.
Information of DNA sequences used to reconstruct phylogenetic trees. Sequences newly generated in this study are indicated in bold.
| Taxon | Voucher ID | ITS | 28S | TEF1 | RPB1 | RPB2 | References |
|---|---|---|---|---|---|---|---|
| Tengioboletus glutinosus | HKAS53425 | – | KF112341 | KF112204 | KF112578 | KF112800 | [22] |
| T. glutinosus | HKAS53452 | – | KT990655 | KT990844 | KT990994 | KT990480 | [5] |
| T. reticulatus | HKAS53426 | – | KF112491 | KF112313 | KF112649 | KF112828 | [22] |
| T. reticulatus | HKAS52241 | – | KT990657 | KT990845 | KT990995 | KT990481 | [5] |
| T. reticulatus | HKAS53453 | – | KT990656 | KT990846 | – | KT990482 | [5] |
| T. funjianensis | HKAS76661 | – | KF112342 | KF112205 | – | KF112801 | [22] |
| T. funjianensis | HKAS77869 | – | KT990658 | KT990847 | KT990996 | KT990483 | [5] |
| T. subglutinosus | HMJAU59034 (T286) | – | OL588198 | OL739119 | OL739121 | – | this study |
| T. subglutinosus | HMJAU59035 (T293) | – | OL588197 | OL739120 | OL739122 | OL739118 | this study |
| Porphyrellus porphyrosporus | MB97-023 | – | DQ534643 | GU187734 | GU187475 | GU187800 | [50] |
| P. porphyrosporus | HKAS76671 | – | KF112482 | KF112243 | KF112611 | KF112718 | [22] |
| Tylopilus sp. | HKAS50211 | – | KT990552 | KT990752 | KT990920 | KT990389 | [22] |
| Tylopilus sp. | HKAS59826 | – | KT990558 | – | – | – | [5] |
| Tylopilus sp. | HKAS90198 | – | KT990559 | – | – | – | [5] |
| Strobilomyces atrosquamosus | HKAS55368 | – | KT990648 | KT990839 | KT990989 | KT990476 | [5] |
| S. atrosquamosus | HKAS78563 | – | KT990649 | KT990833 | KT990983 | KT990470 | [5] |
| S. echinocephalus | HKAS59420 | – | KF112463 | KF112256 | KF112600 | KF112810 | [22] |
| P. aff. alboater | HKAS55375 | – | KT990622 | KT990816 | KT990969 | – | [5] |
| P. nigropurpureus | HKAS74938 | – | KF112466 | KF112246 | – | KF112763 | [22] |
| P. nigropurpureus | HKAS52685 | – | KT990627 | KT990821 | KT990973 | KT990459 | [5] |
| P. nigropurpureus | HKAS53370 | – | KT990628 | KT990822 | KT990974 | KT990460 | [5] |
| P. holophaeus | HKAS50508 | – | KF112465 | KF112244 | KF112553 | – | [22] |
| P. holophaeus | HKAS74894 | – | KF112474 | KF112245 | KF112554 | – | [22] |
| P. castaneus | HKAS63076 | – | KT990548 | KT990749 | KT990916 | KT990386 | [5] |
| P. castaneus | HKAS52554 | – | KT990697 | KT990883 | KT991026 | KT990502 | [5] |
| P. orientifumosipes | HKAS75078 | – | KF112481 | KF112242 | – | KF112717 | [22] |
| P. orientifumosipes | HKAS53372 | – | KT990629 | KT990823 | KT990975 | KT990461 | [5] |
| Boletus bainiugan | HKAS52235 | – | KF112457 | KF112203 | KF112587 | KF112705 | [22] |
| B. bainiugan | HKAS55393 | – | JN563852 | – | JN563868 | – | [51] |
| B. fagacicola | HKAS55975 | – | JN563853 | – | JN563879 | – | [51] |
| B. fagacicola | HKAS71347 | – | JQ172790 | – | JQ172791 | – | [51] |
| Xanthoconium affine | NY00815399 (REH8660) | – | KT990661 | KT990850 | KT990999 | KT990486 | [5] |
| X. porophyllum | HKAS90217 | – | KT990662 | KT990851 | KT991000 | KT990487 | [5] |
| Baorangia pseudocalopus | HKAS63607 | – | KF112355 | KF112167 | – | – | [22] |
| Ba. pseudocalopus | HKAS75081 | – | KF112356 | KF112168 | – | – | [22] |
| Butyriboletus abieticola | Arora11087 | KC184412 | KC184413 | – | – | – | [33] |
| Bu. appendiculatus | Bap1 | KJ419923 | AF456837 | JQ327025 | – | – | [52] |
| Bu. appendiculatus | BR50200893390-25 | KT002598 | KT002609 | KT002633 | – | – | [53] |
| Bu. appendiculatus | BR50200892955-50 | KJ605668 | KJ605677 | KJ619472 | – | KP055030 | [54] |
| Bu. appendiculatus | MB000286 | KT002599 | KT002610 | KT002634 | – | – | [53] |
| Bu. autumniregius | Arora11108 | KC184423 | KC184424 | – | – | – | [33] |
| Bu. brunneus | NY00013631 | KT002600 | KT002611 | KT002635 | – | – | [53] |
| Bu. fechtneri | AT2003097 | KC584784 | KF030270 | – | – | – | [21] |
| Exsudoporus frostii | JLF2548 | KC812303 | KC812304 | – | – | – | [33] |
| E. frostii | NY815462 | – | JQ924342 | KF112164 | – | KF112675 | [22] |
| E. floridanus | BOS 617, BZ 3170 | MN250222 | MK601725 | MK721079 | – | MK766287 | [43] |
| Bu. hainanensis | N.K. Zeng 1197 (FHMU 2410) | KU961653 | KU961651 | – | – | KU961658 | [32] |
| Bu. hainanensis | N.K. Zeng 2418 (FHMU 2437) | KU961654 | KU961652 | KU961656 | – | KX453856 | [32] |
| Bu. huangnianlaii | N.K. Zeng 3245 (FHMU 2206) | MH885350 | MH879688 | MH879717 | – | MH879740 | [28] |
| Bu. huangnianlaii | N.K. Zeng 3246 (FHMU 2207) | MH885351 | MH879689 | MH879718 | – | MH879741 | [28] |
| Bu. peckii | 3959 | – | JQ326999 | JQ327026 | – | – | [55] |
| Bu. persolidus | Arora11110 | KC184444 | – | – | – | – | [33] |
| Bu. primiregius | DBB00606 | – | KC184451 | – | – | – | [33] |
| Bu. fuscoroseus | BR50201618465-02 | KT002602 | KT002613 | KT002637 | – | – | [53] |
| Bu. fuscoroseus | BR50201533559-51 | KT002603 | KT002614 | KT002638 | – | – | [53] |
| Bu. pseudospeciosus | HKAS59467 | – | KF112331 | KF112176 | – | KF112672 | [22] |
| Bu. pseudospeciosus | HKAS63513 | – | KT990541 | KT990743 | – | KT990380 | [5] |
| Bu. pseudospeciosus | HKAS63596 | – | KT990542 | KT990744 | – | KT990381 | [5] |
| Bu. pseudospeciosus | N.K. Zeng 2127 (FHMU 1391) | MH885349 | MH879687 | MH879716 | – | – | [28] |
| Bu. fuscoroseus | MG383a | KC184458 | – | – | – | – | [33] |
| Bu. pulchriceps | DS4514 | – | KF030261 | KF030409 | – | – | [21] |
| Bu. pulchriceps | R. Chapman 0945 | KT002604 | KT002615 | KT002639 | – | – | [53] |
| Bu. querciregius | Arora11100 | KC184461 | – | – | – | – | [33] |
| Bu. regius | MB000287 | KT002605 | KT002616 | KT002640 | – | – | [53] |
| Bu. regius | MG408a | KC584789 | KC584790 | – | – | – | [33] |
| Bu. regius | PRM:923465 | KJ419920 | KJ419931 | – | – | – | [56] |
| Bu. roseoflavus | Arora11054 | KC184434 | KC184435 | – | – | – | [33] |
| Bu. roseoflavus | HKAS63593 | KJ909517 | KJ184559 | KJ184571 | – | – | [53] |
| Bu. roseoflavus | HKAS54099 | KJ909519 | KF739665 | KF739779 | – | – | [53] |
| Bu. roseoflavus | N.K. Zeng 2123 (FHMU 1387) | MH885348 | MH879686 | MH879715 | – | – | [28] |
| Bu. pseudoroseoflavus | HMJAU59470 (T274) | OL604164 | OL587853 | OL739124 | – | OL739126 | this study |
| Bu. pseudoroseoflavus | HMJAU59471 (R383) | OL604165 | OL587852 | OL739123 | – | OL739125 | this study |
| Bu. Roseopurpureus | E.E. Both3765 | KT002606 | KT002617 | KT002641 | – | – | [53] |
| Bu. Roseopurpureus | JLF2566 | KC184466 | KC184467 | – | – | – | [33] |
| Bu. Roseopurpureus | MB06-059 | KC184464 | KF030262 | KF030410 | – | – | [21] |
| Bu. sanicibus | Arora99211 | KC184469 | KC184470 | – | – | – | [33] |
| Bu. subregius | HMJAU60200 (T95) | OM237336 | OM237339 | OM285111 | – | OM285109 | this study |
| Bu. subregius | HMJAU60201 (T198) | OM237337 | OM237340 | OM285112 | – | OM285110 | this study |
| Butyriboletus sp. | MHHNU7456 | – | KT990539 | KT990741 | – | KT990378 | [5] |
| Butyriboletus sp. | HKAS52525 | – | KF112337 | KF112163 | – | KF112671 | [22] |
| Butyriboletus sp. | HKAS57774 | – | KF112330 | KF112155 | – | KF112670 | [22] |
| Bu. hainanensis | HKAS59814 | – | KF112336 | KF112199 | – | KF112699 | [22] |
| Butyriboletus yicibus | HKAS63528 | – | KF112332 | KF112156 | – | KF112673 | [22] |
| Bu. Subappendiculatus | MB000260 | KT002607 | KT002618 | KT002642 | – | – | [53] |
| Bu. subsplendidus | HKAS52661 | – | KF112339 | KF112169 | – | KF112676 | [5] |
| Bu. taughannockensis | 263101 | MH257559 | MH236172 | – | – | – | |
| Bu. taughannockensis | 250839 | MH234472 | MH234473 | – | – | – | |
| Bu. taughannockensis | 252208 | MH236100 | MH236099 | – | – | – | |
| Bu. yicibus | Arora9727 | KC184474 | KC184475 | – | – | – | [33] |
| Bu. yicibus | HKAS57503 | KT002608 | KT002620 | KT002644 | – | – | [53] |
| Bu. yicibus | HKAS68010 | KJ909521 | KT002619 | KT002643 | – | – | [53] |
| Gymnogaster boletoides | NY01194009 (REH9455) | – | KT990572 | KT990768 | – | KT990406 | [5] |
| Rugiboletus brunneiporus | HKAS83209 | – | KM605134 | KM605144 | – | KM605168 | [27] |
| R. extremiorientalis | HKAS63635 | – | KF112403 | KF112198 | – | KF112720 | [22] |
| Crocinoboletus laetissimus | HKAS50232 | — | KT990567 | KT990762 | KT990925 | — | [5] |
| C. laetissimus | HKAS59701 | — | KF112436 | — | — | KF112711 | [22] |
| C. rufoaureus | HKAS53424 | — | KF112435 | KF112206 | KF112533 | KF112710 | [22] |
| C. rufoaureus | HKAS59820 | — | KF112434 | — | KF112532 | KF112709 | [22] |
| Cyanoboletus brunneoruber | HKAS63504 | — | KF112368 | KF112194 | KF112531 | KF112702 | [22] |
| Cy. brunneoruber | HKAS80579 (1) | — | KT990568 | KT990763 | KT990926 | KT990401 | [5] |
| Cy. brunneoruber | HKAS80579 (2) | — | KT990569 | KT990764 | KT990927 | KT990402 | [5] |
| Cy. instabilis | HKAS59554 | — | KF112412 | KF112186 | KF112528 | KF112698 | [22] |
| Cy. pulverulentus | 9606 | — | KF030313 | KF030418 | KF030364 | — | [21] |
| Baorangia pseudocalopus | HKAS63607 | — | KF112355 | KF112167 | KF112519 | KF112677 | [22] |
| Ba. pseudocalopus | HKAS75081 | — | KF112356 | KF112168 | KF112520 | KF112678 | [22] |
| Lanmaoa angustispora | HKAS74765 | — | KF112322 | KF112159 | KF112521 | KF112680 | [22] |
| L. angustispora | HKAS74752 | — | KM605139 | KM605154 | KM605166 | KM605177 | [27] |
| L. angustispora | HKAS74759 | — | KM605140 | KM605155 | KM605167 | KM605178 | [27] |
| L. asiatica | HKAS54094 | — | KF112353 | KF112161 | KF112522 | KF112682 | [22] |
| L. asiatica | HKAS63516 | — | KT990584 | KT990780 | KT990935 | KT990419 | [5] |
| L. fragrans | 18555 | JF907800 | – | – | – | – | |
| Neoboletus brunneissimus | HKAS52660 | — | KF112314 | KF112143 | KF112492 | KF112650 | [22] |
| N. hainanaensis | HKAS63515 | — | KT990614 | KT990808 | KT990964 | KT990449 | [5] |
| N. ferrugineus | HKAS77617 | — | KT990595 | KT990788 | KT990943 | KT990430 | [5] |
| N. ferrugineus | HKAS77718 | — | KT990596 | KT990789 | KT990944 | KT990431 | [5] |
| N. flavidus | HKAS59443 | — | KU974139 | KU974136 | KU974142 | KU974144 | [5] |
| N. flavidus | HKAS58724 | — | KU974140 | KU974137 | KU974143 | KU974145 | [5] |
| Porphyrellus castaneus | HKAS52554 | — | KT990697 | KT990883 | KT991026 | KT990502 | [5] |
| P. castaneus | HKAS63076 | — | KT990548 | KT990749 | KT990916 | KT990386 | [5] |
| P. castaneus | HKAS68575 | — | KT990560 | — | — | — | [5] |
| P. holophaeus | HKAS59407 | — | KT990708 | KT990888 | KT991030 | KT990506 | [5] |
| P. nigropurpureus | HKAS52685 | — | KT990627 | KT990821 | KT990973 | KT990459 | [5] |
| P. nigropurpureus | HKAS53370 | — | KT990628 | KT990822 | KT990974 | KT990460 | [5] |
| P. orientifumosipes | HKAS75078 | — | KF112481 | KF112242 | — | KF112717 | [22] |
| P. orientifumosipes | HKAS53372 | — | KT990629 | KT990823 | KT990975 | KT990461 | [5] |
| Rubroboletus dupainii | JAM0607 | — | — | KF030413 | KF030361 | — | [21] |
| R. latisporus | HKAS63517 | — | KP055022 | KP055019 | KP055025 | KP055028 | [25] |
| R. latisporus | HKAS80358 | — | KP055023 | KP055020 | KP055026 | KP055029 | [25] |
| R. rhodosanguineus | 4252 | — | KF030252 | KF030412 | — | — | [21] |
| R. rhodoxanthus | HKAS84879 | — | KT990637 | KT990831 | KT990981 | KT990468 | [5] |
| R. sinicus | HKAS68620 | — | KF112319 | KF112146 | KF112504 | KF112661 | [22] |
| R. sinicus | HKAS56304 | — | KJ605673 | KJ619483 | KJ619482 | KP055031 | [54] |
| Suillellus amygdalinus | 112605ba | — | JQ326996 | JQ327024 | KF030360 | — | [55] |
| S. amygdalinus | NY00035656 (Thiers54483) | — | KT990650 | KT990840 | KT990990 | KT990477 | [5] |
| S. subamygdalinus | HKAS57262 | — | KF112316 | KF112174 | KF112501 | KF112660 | [22] |
| S. subamygdalinus | HKAS53641 | — | KT990651 | KT990841 | KT990991 | KT990478 | [5] |
| S. subamygdalinus | HKAS57953 | — | KT990652 | KT990842 | KT990992 | — | [5] |
| S. subamygdalinus | HKAS74745 | — | KT990653 | KT990843 | KT990993 | KT990479 | [5] |
| S. lacrymibasidiatus | HMJAU60202 (W3194) | OM237315 | OM230174 | OM285117 | OM285113 | OM285115 | this study |
| S. lacrymibasidiatus | HMJAU60203 (W3229) | OM237338 | OM230172 | OM285116 | – | OM285114 | this study |
| Sutorius eximius | REH9400 | — | JQ327004 | JQ327029 | — | — | [55] |
| Su. eximius | HKAS52672 | — | KF112399 | KF112207 | KF112584 | KF112802 | [22] |
| Su. luridiformis | AT1998054 | UDB000658 | – | – | – | – | |
| Tylopilus alpinus | HKAS55438 | — | KF112404 | KF112191 | KF112538 | KF112687 | [22] |
| Ty. argillaceus | HKAS90201 | — | KT990588 | KT990783 | — | — | [5] |
| Ty. argillaceus | HKAS90186 | — | KT990589 | KT990784 | — | KT990424 | [5] |
| Ty. atripurpureus | HKAS50208 | — | KF112472 | KF112283 | KF112620 | KF112799 | [22] |
| Ty. badiceps | MB03-052 | — | KF030336 | — | — | — | [21] |
| Ty. badiceps | 78206 | — | KF030335 | KF030429 | — | — | [21] |
| Veloporphyrellus alpinus | HKAS68301 | — | JX984538 | JX984550 | — | — | [57] |
| V. alpinus | HKAS57490 | — | KF112380 | KF112209 | KF112555 | KF112733 | [22] |
| V. conicus | BZ2408 | — | JX984545 | — | — | — | [57] |
| V. conicus | BZ1670 | — | JX984543 | JX984555 | — | — | [57] |
| V. gracilioides | HKAS53590 | — | KF112381 | KF112210 | KF112556 | KF112734 | [22] |
| Zangia citrina | HKAS52677 | — | HQ326940 | HQ32687 | — | — | [58] |
| Z. citrina | HKAS52684 | — | HQ326941 | HQ326872 | — | — | [58] |
| Z. erythrocephala | HKAS52843 | — | HQ326943 | — | — | — | [58] |
| Z. erythrocephala | HKAS52844 | — | HQ326944 | — | — | — | [58] |
| Z. olivaceobrunnea | HKAS52275 | — | HQ326947 | HQ326875 | — | — | [58] |
| Z. roseola | HKAS75046 | — | KF112414 | KF112269 | KF112579 | KF112791 | [22] |
| S. luridus | IB2004270 | EF644104 | – | – | – | – | [59] |
| S. luridus | 18902 | JF907802 | – | – | – | – | [60] |
| S. luridus | 18182 | JF907793 | – | – | – | – | [60] |
| S. luridus | Blu3 | AY278765 | – | – | – | – | [61] |
| S. luridus | AMB12636 | KC734542 | – | – | – | – | [13] |
| S. luridus | AMB12638 | KC734544 | – | – | – | – | [13] |
| S. luridus | TL-6877 | AJ889930 | – | – | – | – | |
| S. luridus | TL-6877 | UDB000077 | – | – | – | – | |
| S. luridus | 1968 | AY278766 | – | – | – | – | [61] |
| S. luridus | BL2-VII-10 | JQ685714 | – | – | – | – | [61] |
| S. luridus | AT-04 | UDB002401 | – | – | – | – | |
| S. luridus | UP12 | DQ658866 | – | – | – | – | [62] |
| S. luridus | 17696 | JF907789 | – | – | – | – | [60] |
| S. luridus | BL1-VII-09 | JQ685715 | – | – | – | – | |
| S. luridus | MA-Fungi 47706 | AJ419191 | – | – | – | – | [63] |
| S. mendax | AMB12632 | KC734547 | – | – | – | – | [13] |
| S. mendax | AMB12633 | KC734548 | – | – | – | – | [13] |
| S. mendax | AMB12634 | KC734543 | – | – | – | – | [13] |
| S. mendax | AMB12635 | KC734545 | – | – | – | – | [13] |
| S. mendax | AMB12637 | KC734540 | – | – | – | – | [13] |
| S. mendax | AMB12640 | KC734541 | – | – | – | – | [13] |
| Boletus luridus | UF107 | HM347662 | – | – | – | – | |
| B. amygdalinus | src491 | DQ974705 | – | – | – | – | [64] |
| B. comptus | 17827 | JF907791 | – | – | – | – | [60] |
| B. comptus | AMB12639 | KC734539 | – | – | – | – | [13] |
| B. queletii | 17196 | JF907784 | – | – | – | – | [60] |
| B. queletii | 17208 | JF907785 | – | – | – | – | [60] |
| B. queletii | AMB12641 | KC734546 | – | – | – | – | [13] |
| B. queletii | JV01-231 | UDB000760 | – | – | – | – | |
| N. erythropus | MA-Fungi 47702 | AJ419188 | – | – | – | – | [63] |
| N. erythropus | BOER_TO_2 (AAM630/06) | FM958177 | – | – | – | – | |
| N. erythropus | UF278 | HM347644 | – | – | – | – | |
| N. erythropus | UF276 | HM347643 | – | – | – | – | |
| N. erythropus | UF269 | HM347665 | – | – | – | – | |
| N. erythropus | DG05-54 | UDB001523 | – | – | – | – | |
| N. erythropus | SU46 | DQ131633 | – | – | – | – | [65] |
| N. erythropus | SU47 | DQ131634 | – | – | – | – | [65] |
| N. erythropus | Daniels 582 | AJ496595 | – | – | – | – | [63] |
| Caloboletus calopus | AT1998059 | UDB000659 | – | – | – | – | |
| Ca. radicans | TUF106003 | UDB003224 | – | – | – | – | |
| Bu. fuscoroseus | AH96025 | UDB000649 | – | – | – | – | |
| Bu. fuscoroseus | AT1996017 | UDB000652 | – | – | – | – | |
| Bu. fechtneri | AT2003097 | UDB000703 | – | – | – | – | [21] |
| Imperator rhodopurpureus | AT1996058 | UDB000654 | – | – | – | – | |
| R. pulchrotinctus | GS0860 | UDB000407 | – | – | – | – | |
| R. satanas | AT1998051 | UDB000415 | – | – | – | – | |
| R. rubrosanguineus | GS0405 | UDB000410 | – | – | – | – | |
| R. rhodoxanthus | AT2000182 | UDB001116 | – | – | – | – | |
| Cyanoboletus pulverulentus | RT00004 | EU819502 | – | – | – | – | |
| Cyanoboletus pulverulentus | AH97030 | UDB000408 | – | – | – | – | [66] |
| B. aestivalis | AT2004040 | UDB001113 | – | – | – | – | |
| B. aereus | AT2000198 | UDB000943 | – | – | – | – |