Figure 1.

Infection of BeWo trophoblast-like cells. (a) Adherence and (b) Internalisation ratios, at 2 h post-infection (PI); (c) Intracellular multiplication at 2, 24, and 48 h PI; (d) LDH release (%) induced at 48 h PI. BeWo cells were infected by B. melitensis 16M or Rev1 or by B. suis bv2 (Bs2) at an MOI 1:100 and lysed at selected intervals for CFU counting and supernatant harvesting. Each study was performed in three independent experiments and each datum was obtained in triplicate; results represent the mean ± SD (n = 3) of one representative experiment. PLSD test: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 vs. other strains of the same experiment.