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. 2022 Mar 2;10(3):549. doi: 10.3390/microorganisms10030549

Table 2.

Substrate specificities of selected bacterial aldoxime dehydratases toward plant oximes.

Enzyme 1 Substrate 2 Relative Activity (%) Vmax [U/mg] KM [mM] Vmax/KM [U/mg/mM]
OxdB
[14]		 IAOx 18 5.42 2.40 2.26
iVaOx 20 7.72 3.58 2.16
Z-PAOx 100 19.5 0.872 22.4
Z-PPOx 63 14.3 1.36 10.5
OxdK
[21]		 IAOx 1 n.d. n.d. n.d.
iBuOx 14 5.87 0.538 10.9
iVaOx 100 35.1 1.33 26.4
Z-PAOx 7 2.61 0.991 2.63
Z-PPOx 31 12.1 0.975 12.4
OxdRG
[22]		 IAOx 11 0.281 3.91 0.072
iBuOx 39 0.041 5.54 0.007
iVaOx 87 0.239 3.97 0.060
Z-PAOx 40 0.140 1.40 0.100
Z-PPOx 100 0.392 2.31 0.170

1 See Table 1. 2 IAOx, indole-3-acetaldoxime; iBuOx, isobutyraldoxime; iVaOx, isovaleraldoxime (3-methylbutyraldoxime); PAOx, phenylacetaldoxime; PPOx, 3-phenylpropionaldoxime. The substrates are of E/Z-configuration unless noted otherwise. Substrates with the highest relative activity and Vmax/KM are in bold.