Table 3.
Distribution of aldoxime dehydratases, nitrilases, and nitrile hydratases in bacteria.
| Phylum | Genus | Number of Sequences 1 | Number of Active Strains 2 | ||
|---|---|---|---|---|---|
| Oxd | NLase | NHase | |||
| Actinobacteria | Arthrobacter | 5 | 16 | 5 | n.d. |
| Cellulomonas | - | 10 | - | n.d. | |
| Corynebacterium | 3 | 3 | - | 2 | |
| Brevibacterium | 1 | 41 | 1 | n.d. | |
| Gordonia | 4 | 6 | 13 | n.d. | |
| Microbacterium | 5 | 27 | 9 | 1 | |
| Nitriliruptor | 2 | 3 | 1 | n.a. | |
| Micrococcus | - | - | - | 1 | |
| Nocardia | 1 | 36 | 7 | 2 | |
| Rhodococcus | 142 | 190 | 107 | 14 | |
| Streptomyces | 8 | 157 | 252 | n.d. | |
| Firmicutes | Bacillus | 2 | 15 | 2 | 2 |
| Brevibacillus | 2 | 66 | - | n.a. | |
| Cytobacillus | 1 | 1 | - | n.a. | |
| Geomicrobium | 1 | 1 | - | n.a. | |
| Paenibacillus | 1 | 99 | 13 | n.a. | |
| Siminovitchia | 3 | 14 | - | n.a. | |
| Proteobacteria | Afipia | 2 | - | 14 | n.a. |
| Agrobacterium | 9 | 99 | 43 | n.d. | |
| Bradyrhizobium | 248 | 101 | 298 | n.a. | |
| Erwinia | 1 | 19 | 5 | n.d. | |
| Gibbsiella | 1 | 10 | 2 | n.a. | |
| Herbaspirillum | 1 | 45 | 9 | n.a. | |
| Klebsiella | - | 17 | 66 | n.d. | |
| Novosphingobium | 1 | 68 | 1 | n.a. | |
| Paraburkholderia | 1 | 336 | 139 | n.a. | |
| Proteus | - | 1 | 2 | n.d. | |
| Pseudomonas | 48 | 261 | 50 | 2 | |
| Pseudomaricurvus | 1 | - | - | n.a. | |
| Rhizobium | 7 | 270 | 600 | n.a. | |
| Serratia | - | 4 | 11 | n.d. | |
| Variovorax | 73 | 156 | 92 | n.d. | |
| Xanthomonas | 2 | 1 | 2 | n.d. | |
| Bacteroidetes 3 | Flavobacterium | - | 172 | 4 | n.d. |
| Maribacter | 1 | 58 | - | n.a. | |
1 Retrieved from [26]. Searches for hypothetical Oxds were performed using OxdBr1 (WP_044589203 [24]) as a template. Searches for hypothetical NLases and NHases were performed using the NLase from Bradyrhizobium diazoefficiens (NP_770037 [34]) and the α-subunit of the NHase from Rhododoccus erythropolis N-771 (2AHJ_A [42]) as templates, respectively. 2 According to [35]. The active strains exhibited both aldoxime dehydratase and nitrile-hydrolyzing activity for phenylacetaldoxime and phenylacetonitrile, respectively; n.a., not assayed; n.d., not detected. 3 Other four Oxds were identified in a bacterium of the Chitinophagaceae family in Bacteroidetes.