Skip to main content
. 2022 Mar 7;13(3):468. doi: 10.3390/genes13030468

Figure 2.

Figure 2

miR-185-5p overexpression inhibits inflammation and phagocytosis. (A) The transfection efficiency in RAW264.7 macrophages with 50 nM miR-185-5p mimics transfections vs. miR NC by RT-qPCR. (B) Gene expressions (RT-qPCR) and (C) cytokine secretions (ELISA) in LPS-induced RAW264.7 macrophages transfected with 50 nM miR-185-5p mimics or miR NC. (D) Representative images showing phagocytosis of fluorescein-labeled E. coli bioparticles (FITC-Green, DAPI-Blue) in RAW264.7 macrophages with 50 nM miR-185-5p mimics or miR NC transfection and 100 ng/mL LPS for 24 h. The scale bars correspond to 100 μm. (E) Quantification of mean fluorescence intensity of phagocytosis. Mean ± SD; n = 3–6; * p < 0.05; ** p < 0.01; *** p < 0.001.